Gaspard HUBER

Fonction

Researcher in CEA

Thema of research

Developments in the field of hyperpolarization to increase the sensitivity of Nuclear Magnetic Resonance

• Parahydrogen as a source of hyperpolarization

  • ANR Research Project SOFTNMR – Sensitive online flow NMR techniques

    One PhD student fellow starting in October 2024.

• Noble gases polarized by optical pumping

  • ANR Research Project HELPING – High field enhancement of nuclear polarisation in noble gases

Scientific partner. One post-doc fellow at LSDRM.

One PhD student fellow in laboratoire Kastler Brossel, in which G. TASTEVIN coordinates the project.

• Metabolic profiling by NMR

CV

2006	Habilitation to lead research
2002 –	Researcher in CEA (IRAMIS / NIMBE / LSDRM)
1999 – 2001	Researcher in Metabolix Explorer
1998 – 1999	Post-doctoral position in the laboratory BIP-CNRS of Marseille, France, Supervisor Dr. Françoise Guerlesquin
1996 – 1998	Post-doctoral position in the laboratory of NMR, university of Florence, Italy, Supervisor Pr. Ivano Bertini
1996	Ph-D thesis
1993 – 1996	Ph-D thesis in the service of Inorganic and Biological Chemistry of CEA, Grenoble, France, Supervisor Dr. Jacques Gaillard
1993	Chemical Engineering degree of ESCIL, now CPE, Lyon

Scientific publications

Interplay of structure and photophysics of individualized rod-shaped graphene quantum dots with up to 132 sp² carbon atoms
D. Medina-Lopez, T. Liu, S. Osella, H. Levy-Falk, N. Rolland, C. Elias, G. Huber, P. Ticku, L. Rondin, B. Jousselme, D. Beljonne, J.-S. Lauret, S. Campidelli, Nat. Comm., 2023, 14, 4728.

Methods Based on Solution Flow, Improved Detection and Hyperpolarization for Enhanced Magnetic Resonance
P. Berthault and G. Huber, chapter 6, ‘Magnetic Resonance Microscopy’, Wiley-VCH, 2022.
Open access link: https://cea.hal.science/cea-04383640

General Guidelines for Sample Preparation Strategies in HR‐μMAS NMR based Metabolomics of Microscopic Specimens
C. Lucas‐Torres, T. Bernard, G. Huber, P. Berthault, Y Nishiyama, PS Kandiyal, B Elena‐Herrmann, L Molin, F Solari, A-K Bouzier‐Sore, A Wong, Metabolites, 2020, 10, 54.
Open access link: https://hal.archives-ouvertes.fr/cea-02464528

Cucurbit[5]uril derivatives as oxygen carriers
G Huber, P Berthault, AL Nguyen, A Pruvost, E Barruet, J Rivollier, M-P Heck, B Prieur, Supramol. Chem., 2019, 31, 668-675.
Open access link: https://hal-cea.archives-ouvertes.fr/cea-02273187/document

Single-scan diffusion-ordered NMR spectroscopy of SABRE-hyperpolarized mixtures
L Guduff, P Berthault, C van Heijenoort, J-N Dumez, G Huber, ChemPhysChem, 2019, 20, 392-398.

HR-μMAS NMR-based metabolomics: localized metabolic profiling of a garlic clove with μg tissues
C Lucas-Torres, G Huber, A Ichikawa, Y Nishiyama, A Wong, Analytical Chemistry, 90, 13736-13743.

Functionalization of Bambusurils by thiol-ene click reaction and new, facile method for the preparation of anion-free Bambus[6]urils
D Azazna, M Lafosse, J. Rivollier, J Wang, I Ben Cheikh, M Meyer, P Thuéry, J-P Dognon, G, Huber, M-P Heck, Chem. Eur. J., 2018, 24, 10793-10801.

Unsaturated cryptophanes: toward dual PHIP/hyperpolarised xenon sensors
G Huber, E Léonce, O Baydoun, N De Rycke, T Brotin, P Berthault, Magn Reson Chem, 2017, 1-7.

Metabolomic and proteomic investigations of impacts of titanium dioxide nanoparticles on Escherichia coli
M Planchon, T Léger, O Spalla, G Huber, R Ferrari, Plos One, 2017.

Single-scan multidimensional NMR analysis of mixtures at sub-millimolar concentrations using SABRE hyperpolarization
V Daniele, F-X Legrand, P Berthault, J-N Dumez, G Huber, ChemPhysChem, 2015, 16, 3413-3417.

Nuclear spin noise in NMR revisited,
G Ferrand, G Huber, M Luong, H Desvaux, J. Chem. Phys., 2015, 143, 094201.

A more accurate tuning-matching technique for NMR probes using wobulation and variable phase shifter,
G Ferrand, M Luong, G Huber, H Desvaux, Concepts Magn. Reson. B, 2015, 45B, 59.

On the tuning of high resolution NMR probes,
M T Pöschko, J Schlagnitweit, G Huber, M Nausner, M Horničáková, H Desvaux, N Müller, ChemPhysChem, 2014, 15, 3639.

Synthesis of cucurbit[6]uril derivatives and insights into their solubility in water,
V Lewin, J Rivollier, S Coudert, A Buisson, D Baumann, B Rousseau, F-X Legrand, H Kourilová, P Berthault, J-P Dognon, M-P Heck, G Huber, Eur. J. Org. Chem., 2013, 3857.

Multiple echoes due to distant dipolar fields in NMR of hyperpolarized noble gas solutions,
S W Morgan, E Baudin, G Huber, P Berthault, G Tastevin, M Goldman, P-J Nacher, and H Desvaux, Eur. Phys. J. D., 2013, 67, 29.

Cell uptake of a biosensor detected by hyperpolarized 129Xe NMR: The transferrin case,
C Boutin, A Stopin, F Lenda, T Brotin, J-P Dutasta, N Jamin, A Sanson, Y Boulard, F Leteurtre, G Huber, A Bogaert-Buchmann, N Tassali, H Desvaux, M Carrière, and P Berthault, Bioorg. Med. Chem., 2011, 19, 4135-4143.

Interaction of Xenon with Cucurbit[5]uril in Water,
G Huber, F-X Legrand, V Lewin, D Baumann, M-P Heck, P Berthault, ChemPhysChem, 2011, 1053-1055.

A water-soluble Xe@cryptophane-111 complex exhibits very high thermodynamic stability and a peculiar ¹²⁹Xe NMR chemical shift, RM Fairchild, AI Joseph, KT Holman, HA Fogarty, T Brotin, J-P Dutasta, C Boutin, G Huber, P Berthault, J. Am. Chem. Soc., 2010, 132, 15505-15507.

Biosensing using laser-polarized xenon NMR/MRI,
P Berthault, G Huber, H Desvaux, Prog. NMR Spectrosc., 2009, 55, 35-60.

Nuclear spin-noise spectra of hyperpolarized systems,
H Desvaux, D J-Y Marion, G Huber, P Berthault, Angew. Chem. Int. Ed. 2009, 48, 4341-4343.

Towards thrombosis-targeted zeolite nanoparticles for laser-polarized ¹²⁹ Xe MRI,
F Lerouge, O Melnyk, J-O Durand, L Raehm, P Berthault, G Huber, H Desvaux, A Constantinesco, P Choquet, J Detour, M Smaïhi, J. Mater. Chem, 2009, 19, 379-386.

Sensitivity and multiplexing capabilities of MRI based on hyperpolarized ¹²⁹Xe biosensors,
P Berthault, A Bogaert, H Desvaux, G Huber, Y Boulard, J. Am. Chem. Soc., 2008, 130, 16456-16457.

Cryptophane-xenon complexes in organic solvents observed through NMR spectroscopy,
G Huber, L Beguin, H Desvaux, T Brotin, H A Fogarty, J-P Dutasta, P Berthault, J. Phys. Chem. A, 2008, 112, 11363-11372.

Observation of noise-triggered chaotic emissions in an NMR-maser,
D J-Y Marion, G Huber, P Berthault, H Desvaux, ChemPhysChem, 2008, 9, 1395-1401.

Effects on ¹ H and ¹²⁹ Xe NMR spectra of large magnetization created by dissolved laser-polarized xenon,
D J-Y Marion, G Huber, P Berthault, H Desvaux, C. R. Chimie, 2008, 11, 553-559.

A cryptophane core optimized for xenon encapsulation,
H A Fogarty, P Berthault, T Brotin, G Huber, H Desvaux, J-P Dutasta, J. Am. Chem. Soc., 2007, 129, 10332-10333.

A cryptophane biosensor for detection of specific nucleotide targets through xenon-NMR,
V Roy, T Brotin, J-P Dutasta, M-H Charles, T Delair, F Mallet, G Huber, H Desvaux, Y Boulard, P Berthault, ChemPhysChem, 2007, 8, 2082-2085.

¹H and ¹²⁹ Xe NMR absorption line shapes in the presence of highly polarized and concentrated xenon solutions in high magnetic field,
D J Marion, G Huber, L Dubois, P Berthault, H Desvaux, J. Magn. Reson., 2007, 187, 78-87.

Direct enhancement of any solution NMR signal using the distant dipolar fields created by highly polarized and concentrated nuclear spin systems,
H Desvaux, D J Marion, G Huber, L Dubois, P Berthault, Eur. Phys. J. Appl. Phys., 2006, 36, 25–34.

Water soluble cryptophanes showing unprecedented affinity for xenon: candidates as NMR-based biosensors,
G Huber, T Brotin, L Dubois, H Desvaux, J-P Dutasta, P Berthault, J. Am. Chem. Soc., 2006, 128, 6239-6246.

Study of the hydrophobic cavity of β-cryptogein through laser-polarized xenon NMR spectroscopy,
P Berthault, G Huber, P T Ha, L Dubois, H Desvaux, E Guittet, ChemBioChem, 2006, 7, 59-64.

Dynamics of xenon binding inside the hydrophobic cavity of pseudo wild-type bacteriophage T4 lysozyme explored through xenon-based NMR spectroscopy,
H Desvaux, L Dubois, G Huber, M L Quillin, P Berthault, M W Matthews, J. Am. Chem. Soc., 2005, 127, 11676-11683.

Regioselective one-step synthesis of hexahydroxy permethylated β-cyclodextrin and unambiguous NMR analysis,
Y Chen, JG Huber, Zhang Y et Sinaÿ P, C.R. Chimie, 2005, 8, 27-30.

Probing hydrophobic cavities of lipid transfer protein from Nicotiana tabacum though Xenon-based NMR,
L Dubois, Da Silva P, Landon C, JG Huber, Ponchet M, Vovelle F, P Berthault, H Desvaux, JACS, 2004, 126, 15738-15746.

NMR study of optically active monosubstituted cryptophanes and their interaction with xenon,
JG Huber, L Dubois, H Desvaux, T BrotinT, J-P Dutasta, P Berthault, J. Phys. Chem. A, 2004, 108, 9608-9615.

Diisobutylaluminum hydride as a molecular scalpel: the regioselective stripping of four methyl groups from permethylated b-cyclodextrin,
X Luo, Y Chen, JG Huber, Y Zhang, P Sinaÿ, C.R. Chimie, 2004, 7, 25-28.

Mapping hydrophobic molecular regions using dissolved laser-polarized xenon NMR,
L Dubois, P Berthault, JG Huber, H Desvaux, C. R. Physique, 2004, 5, 305-313.

Dynamics of xenon inside cavities as probed by NMR relaxation of dissolved laser-polarized xenon,
L Dubois, S Parrès, JG Huber, P Berthault, H Desvaux, J Phys Chem B, 2004, 108, 767-773.

Magnetization transfer from laser-polarized xenon to protons with spin-diffusion quenching,
H Desvaux, JG Huber, T Brotin, J-P Dutasta, P Berthault, Chem Phys Chem, 2003, 4, 384-387.

Protein-hydration and location of water molecules in oxidized horse heart cytochrome c by (1)H NMR,
I Bertini, JG Huber, C Luchinat, M Piccioli, J Magn Reson, 2000, 147, 1-8.

Solution structure of reduced horse heart cytochrome c,
L Banci, I Bertini, JG Huber, GA Spyroulias, P Turano, J. Biol. Inorg. Chem., 1999, 4, 21-31.

Unusual NMR, EPR, and Mossbauer properties of Chromatium vinosum ,
P Kyritsis, R Kümmerle, JG Huber, J Gaillard, B Guigliarelli, C Popescu, E Münck, J-M Moulis, Biochemistry, 1999, 38, 6335-6345.

Partial orientation of oxidized and reduced cytochrome b ₅ at high magnetic fields : magnetic susceptibility anisotropy contributions and consequences for protein solution structure determination,
L Banci, I Bertini, JG Huber, C Luchinat, A Rosato, J. Am. Chem. Soc., 1998, 120, 12903-12909.

ePHOGSY experiments on a paramagnetic protein : location of the catalytic water molecule in the heme crevice of the oxidized form of horse heart cytochrome c.
I Bertini, C Dalvit, JG Huber, C Luchinat, M Piccioli, FEBS letters, 1997, 415, 45-48.

Intramolecular electron transfer between [4Fe-4S] clusters studied by PMR spectroscopy,
P Kyritsis, JG Huber, I Quinkal, J Gaillard, J-M Moulis, Biochemistry, 1997, 36, 7839-7846.

Use of ¹ H longitudinal relaxation times in the solution structure of paramagnetic proteins. Applications to [4Fe-4S] proteins.
JG Huber, J-M Moulis, J Gaillard, Biochemistry, 1996, 35, 12705-12711.

The GC-MS analysis of organic intermediates from the TiO₂ photocatalytic treatment of water contaminated by lindane (1a, 2a, 3b, 4a, 5a, 6b-hexachlorocyclohexane),
C Guillard, P Pichat, G Huber, C Hoang-Van, J. of Adv. Oxidation technologies, 1996, 1, 53-60.

NMR of Chromatium vinosum ferredoxin : evidence for structural inequivalence and impeded electron transfer between the two [4Fe-4S] clusters.
JG Huber, J Gaillard, J-M Moulis, Biochemistry, 1995, 34, 194-205.

Use of the TiO₂-UV system to detoxify water contaminated by lindane (1α, 2α, 3ß, 4α, 5α, 6ß-Hexachlorocyclohexane),
C Guillard, G Huber, P Pichat, C Hoang-Van, Soil & Environment 1995, 5, 1233.

Patents

Cucurbituril derivatives as oxygen carriers,
B Prieur, M-P Heck, G Huber, owner B Prieur, 2017. EP17305480

Characterization of rubbers and rubber derivatives by NMR,
JG Huber, B Gonzalez, L d’Auriol, owner Metabolic Explorer, 2001. FR2821430 WO02068946-A,1.

Coupling device between a reactor and a NMR spectrometer,
B Gonzalez, JG Huber, L d’Auriol, owners Metabolic Explorer, Bruker SA, 2000. FR2816713, EP 1342097, WO/2002/041021.

Method and apparatus for analyzing the chemical state of living cells by nuclear magnetic resonance,
B Gonzalez, JG Huber, M Piotto, M Renaud, owners Université d’Auvergne, BRUKER SA, 2000. FR2815357, EP1410055, US20030199751, WO/2002/031523.