LIONS (Interdisciplinary Laboratory on Nanoscale and Supramolecular Organization) activities focus on the understanding and utilization of self-assembly for creating supramolecular architectures. The LIONS approach is truly interdisciplinary since it blends statistical physics, experimental physics both in the laboratory (microscopy, scattering, spectroscopy) and on large facilities (neutron scattering, synchrotron sources), chemistry (synthesis, elemental analysis) and biological inspiration. The paramount importance of interfaces in self-assembly means that a lot of effort is devoted to explain the effect of ions at interfaces, how interfaces can be modified between oil and water (stimulable emulsions, microemulsions) or how chemical reactions can transfer species through a liquid-liquid interface (with the help of many microfluidics devices). Elemental bricks for self-assembly are very often nano-objects of various shapes (spheres, tubes or rods) whose nucleation and growth is precisely controled by surface chemistry and kinetically analyzed by time-resolved scattering techniques and statistical physics tools. For some nanoparticles, LIONS has also gained a valuable expertise on their toxicity. At a larger scale, the self-assembly of nanometric bricks is studied for providing nanoparticle networks, supramolecular ligands for membrane attachment, polymer membranes or bio-inspired materials like in studies about biomineralization. LIONS activities are pursued through numerous french and international collaborations (USA, Spain, Italy, Germany, Greece, Israël).