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Time-resolved techniques for the study of biological molecules
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The specificity of the group is the use of high-performing time-resolved spectroscopy. DNA and its constituents are fragile systems so particular care is taken with regards to excitation energy and sample handling.

Time-resolved fluorescence (fluorescence decays, fluorescence anisotropy decays and time-resolved fluorescence spectra) can be recorded from 100 fs to 100 ns using a combination of two detection techniques; fluorescence upconversion and time-correlated single photon counting. An important asset is the use as the same excitation source (a femtosecond titanium:sapphire laser) in both cases.

Nanosecond flash photolysis allows the monitoring of transient states and species from 10 ns to 100 ms. A specific features of our setup is the detection of weak signals (10-4 en absorbance).

 

Maj : 26/01/2016 (1676)

 

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