|IRAMIS, "Saclay Institute of Matter and Radiation", gathers
6 Units or Laboratories with scientific research activities on Condensed Matter, Atoms and Molecules. Most units are associated with other partners : CNRS , École
Polytechnique and ENSICAEN.
Our research topics:
- Radiation-matter interaction
- Materials and complex systems
Phase Separation and Nanodomain Formation in Hybrid Polymer/ Lipid Vesicles
Phase separation and nanodomain formation in hybrid polymer/lipid vesicles
T. P. Tuyen Dao, F. Fernandes, M. Er-Rafik, R. Salva, M. Schmutz, A. Brûlet, M. Prieto, O. Sandre and J.-F. Le Meins, ACS Macro Lett. 4 (2015) 182.
Hybrid polymer/lipid large unilamellar vesicles (LUVs) were studied by small angle neutron scattering (SANS), time-resolved Förster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM). For the first time in hybrid vesicles, evidence for phase separation at the nanoscale was obtained, leading to the formation of stable nanodomains enriched either in lipid or polymer. This stability was allowed by using vesicle-forming copolymer with a membrane thickness close to the lipid bilayer thickness, thereby minimizing the hydrophobic mismatch at the domain periphery. Hybrid giant unilamellar vesicles (GUVs) with the same composition have been previously shown to be unstable and susceptible to fission, suggesting a role of curvature in the stabilization of nanodomains in these structures.
A. Menelle, 2015-01-27