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Sujet de stage / Master 2 Internship

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Miniaturisation of an early diagnostic test based on GMR sensors

Contact: JASMIN-LEBRAS Guenaelle, , guenaelle.jasmin-lebras@cea.fr, +33 1 69 08 65 35
Summary:
The aim of this project is to miniaturise the acquisition and signal processing part of a GMR sensor-based biochip so that it can be developed as a Point of Care diagnostic test.
Possibility of continuation in PhD: Non
Deadline for application:30/04/2022

Full description:
The development of early diagnosis techniques that are fast, sensitive, transportable to the patient's bedside and inexpensive is a challenge in the field of health, but also in the field of defense or the environment. The World Health Organisation has defined criteria (ASSURED) that field diagnostic tests must meet, which proves the real need in terms of public health. The global pandemic of the last year has also shown the need for such tests. In this context, we propose a patented biochip, based on GMR (Giant MagnetoResistance) sensors arranged face to face on either side of the channel, to detect biological objects (bacteria, cells) in very small quantities, in complex matrices without a prior washing step. The proposed approach is very innovative. It is based on the use of magnetic nanoparticles functionalized by monoclonal antibodies produced at the LERI (Laboratoire d'Etudes et Recherches en Immunoanalyse), directed against the target biological objects. The dynamic detection of the latter, after interaction with the magnetic nanoparticles, is carried out using giant magnetoresistance sensors (GMR sensors) developed at the LNO (Nanomagnetism and Oxides Laboratory), which make it possible to count the magnetically marked biological objects one by one. The results are very promising since we are reaching sensitivities equivalent to those obtained on the same biological model with an ELISA test or by flow cytometry. There is a need to make this diagnostic test transportable, initially in high biohazard buildings but also to laboratories, or later at the bedside and in the field.

During this internship, with our colleagues from LETS (CEA/SPEC), the student will miniaturize the acquisition and signal processing part. The planned assembly will include an ARM STM32 embedded processor, the digitization of the signals will use 16-bit analogue to digital converters. The signal processing will be developed on a microcontroller or DSP or even FPGA.

The coincidence analysis program (signals obtained simultaneously by the sensors arranged face to face on either side of the channel) will be executed in real time in flow mode on the signals. It will be tested using the data files obtained from the GMR microarray experiments on bacteria. The system should be developed to run connected to a PC via a USB port, or in stand-alone mode with a screen to display the results.

The student will also have to consider the design of a new compact homogeneous permanent magnet to make the biochip easily transportable.
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