Scanning electron microscopy in a microfluidic cell. Instrumental and methodological developments
|Contact: CHEVALLARD Corinne, , email@example.com, +33 1 69 08 52 23|
The intern will realize proof of concept SEM observations in liquid, by adapting an existing fluidic setup to the specificities of scanning electron microscopy and will develop protocols for image acquisition. This will provide a new tool to in situ and operando studies in liquid media.
|Possibility of continuation in PhD: Non|
|Deadline for application:31/05/2022 |
|Full description: |
Emerging operando microscopies performed in liquid media represent a significant breakthrough since they enable to observe the dynamics of chemical and biological systems in a state very similar to their functional one. In this context we recently have developed a microfluidic cell allowing us to image nanometer-scale objects in water relying on scanning transmission X-ray microscopy (STXM). In addition to morphological information, this technique also provides the nature and on the coordination of the atoms present in each pixel. Unfortunately, STXM instruments are poorly accessible to the scientific community because they must be installed on synchrotron X-ray sources, the only ones that are bright enough for this type of approach. Thus, we have initiated the adaptation of the microfluidic cell to scanning electron microscopy (SEM), a technique comparable in terms of spatial resolution and chemical analysis but which is much more widespread.
The objective of this internship is to realize proof of concept SEM observations of the evolution of a solid sample submitted to a continuous flow of reactive solution. First, the trainee will adapt the existing fluidic setup to the specificities of electron microscopy (e.g. vacuum level, optics arrangement, and charge accumulation). We here anticipate the use of microfabrication (for instance to mold conductive elastomeric gaskets). Second, protocols will be established to inject the chemical solutions and to record the expected events at the solid surface; for example, it will require the characterization of the fluid exchange times, of the spatial resolution, and of elemental analysis modalities. To achieve these goals dedicated substrates with micropatterned metallic films will have to be prepared.
Required profiles: engineering or Master 2 students
Reference: C. Gosse, S. Stanescu, […] and C. Chevallard. A pressure-actuated flow cell for soft X-ray spectromicroscopy in liquid media. Lab Chip, 2020, 20, 3213-3229.
Locations: LIONS, IRAMIS/NIMBE, CEA-Saclay, Gif-sur-Yvette
and HERMES beamline, SOLEIL synchrotron, St-Aubin
Contacts : firstname.lastname@example.org ; email@example.com
|Technics/methods used during the internship: |
Electron microscopy, microfluidics, microfabrication
|Tutor of the internship |
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