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Univ. Paris-Saclay
Frédéric Gobeaux

Frédéric GOBEAUX

Service Nanoscience et Innovation pour les Matériaux, la Biomédecine et l'Energie (NIMBE), UMR 3685

Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supra Moléculaire (LIONS)

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ORCID iD: 0000-0003-4410-2378

GoogleScholar profile

Status

CNRS Research associate / Chargé de recherche au CNRS

Research interests

I am interested in the supramolecular assemblies of biological or biomimetic molecules. For instance, my PhD thesis work was devoted to understanding the physico-chemical conditions that rule type I collagen self-assembly in vitro. We could build biomaterials mimicking biomaterials such as skin or bone, whose main component in vivo is collagen. I have also studied the amyloid-like assembly of short peptides.

More recently, in collaboration with researchers from the Institut Galien and from INSERM, I have focused on the interaction between nanoparticles and proteins in order to better understand the pharmacodynamics of nanodrugs.

Overall, my goals are to understand the kinetics and mechanisms of assembly from the atomic level up to the supramolecular level i.e., from the ångström to the millimeter. This wide range of scales requires combining different techniques of structural characterization: (cryo)transmission electron microscopy, radiation scattering (x-ray and neutron) and vibrational spectroscopies. I also occasionally use microfluidics tools.

These tools led me to expand my fields of interest to mineral systems.

For example, I use SAXS and polarized light microscopy to characterize the nanostructure of lead-based paint mediums. This work is done in collaboration with Laurence de Viguerie from the LAMS who reconstitutes paint formulations from old painters's recipes.

Likewise, cryogenic transmission electron microscopy is also a very useful tool to characterize clay minerals like imogolite nanotubes (with Antoine Thill) and to quench intermediate species during mineral precipitation (projects with Corinne Chevallard and David Carrière).

Lead soap observed between cross-polarizers. Formulation by Lucie Laporte (LAMS)

Keywords: soft matter, self-assembly, liquid crystals, colloidal nanoassemblies, nanoparticles


Mes travaux de recherche s'intéressent principalement à l’étude physico-chimique d’auto-assemblages biologiques ou biomimétiques. Par exemple, au cours de mon travail de thèse j’ai étudié différents aspects des auto-assemblages du collagène de type I, une protéine structurale majeure des tissus conjonctifs chez les vertébrés. J'ai aussi étudié les assemblages peptidiques de type amyloïde.

Mes objectifs sont de contrôler les conditions physico-chimiques et de comprendre les mécanismes d’auto-assemblage depuis le niveau atomique jusqu’au niveau supramoléculaire (de l’angström jusqu’au millimètre). Pour ce faire, je combine différentes techniques de caractérisation : microscopies (optique et électronique), diffusion du rayonnement (SAXS, SANS) et spectroscopies vibrationnelles (infrarouge, dichroïsme circulaire, Raman). Je me suis aussi intéressé au développement d’outils microfluidiques pour la cristallisation des protéines.

Previous positions/Education

2009-2012

 CEA Saclay - iBiTec-S, SB2SM, URA 2096

Institut de Physique de Rennes UMR 6251, Équipe Biophysique

Supervision: Dr. Maïté Paternostre & Dr. Franck Artzner

2007-2009

Brandeis University, Complex Fluids Group (Waltham, MA, USA)
Supervision: Prof. Seth Fraden

2004-2007

Thèse de doctorat de l’Université Pierre et Marie Curie (Paris 6)

Phases denses de collagène de type I : transition isotrope/cholestérique, firillogenèse et minéralisation

Dirigée par : Dr. Gervaise Mosser Laboratoire de Chimie de la Matière Condensée de Paris (UMR 7574)

                    Dr. Patrick Davidson Laboratoire de Physique du Solide (UMR 8502)

2001-2004 École Nationale Supérieure de Chimie de Paris (Chimie ParisTech)

 

Publications

Most manuscripts are available at the HAL depository (since 2014) https://hal.archives-ouvertes.fr/ or upon request (before 2014).

Les manuscrits (2014-présents) sont disponibles sur le serveur HAL https://hal.archives-ouvertes.fr/ ou sur demande (avant 2014).

 

Nanomedicine

Taken from Dormont et al. (2019)

"New nanoparticle formulation for cyclosporin A: In vitro assessment" A. Gendron, N. Lan Linh Tran, J. Laloy, R. Brusini, A. Rachet, F. Gobeaux, V. Nicolas, P. Chaminade, S. Abreu, D. Desmaële, M. Varna Pharmaceutics 2021 13(1) 91

"Albumin-driven disassembly of lipidic nanoparticles: the specific case of the squalene-adenosine nanodrug" F. Gobeaux*, J. Bizeau, F. Samson, L. Marichal, I. Grillo, F. Wien, S. O. Yesylevsky, C. Ramseyer, M. Rouquette, S. Lepêtre-Mouelhi, D. Desmaële, P. Couvreur, P. Guenoun, J.-P. Renault, F. Testard Nanoscale 2020 12 2793-280910

"Translation of Nanomedicines from Lab to Industrial Scale Synthesis: The Case of Squalene-Adenosine Nanoparticles" F. Dormont, M. Rouquette, C. Mahatsekake, F. Gobeaux, R. Brusini, S. Calet, F. Testard, S. Lepetre-Mouelhi, D. Desmaële, M. Varna, P. Couvreur Journal of Controlled Release 2019 307 302-314

"Towards a clinical application of freeze-dried squalene-based nanomedicines" M. Rouquette, K. Ser-Le Roux, M. Polrot, C. Bourgaux, J.-M. Michel, F. Testard, F. Gobeaux, S. Lepetre-Mouelhi Journal of Drug Targeting 2019 699-708

 

 

 

Imogolite Nanotubes Structure and Functionalization (cryo-TEM characterization)

Imogolite suspension
​​​​​observed by cryoTEM

"Tailoring structure and surface chemistry of hollow allophane nanospheres for optimization of aggregation by facile methyl modification" P. Du, A. Thill, P. Yuan, S. Wang, D. Liu, F. Gobeaux, L. Deng, Y. Song Applied Surface Science 2020 510 145453

"Dual internal functionalization of imogolite nanotubes as evidenced by optical properties of Nile red" P. Picot, F. Gobeaux, T. Coradin, A. Thill Applied Clay Science 2019 178 105133

"Partial Transformation of Imogolite by Decylphosphonic Acid Yields an Interface Active Composite Material" T. Lange, T. Charpentier, F. Gobeaux, S. Charton, F. Testard, A. Thill Langmuir 2019 35, 11, 4068-4076

"Exploring hybrid imogolite nanotubes formation via Si/Al stoichiometry control" P. Picot, Y. Liao, É. Barruet, F. Gobeaux, T. Coradin, A. Thill Langmuir 2018 34 13225-13234

 

 

 

 

Amyloid Fibrils Reversible Assembly

Taken from Gobeaux & Wien (2018)

"Reversible Assembly of a Drug Peptide into Amyloid Fibrils: A Dynamic Circular Dichroism Study" F. Gobeaux* & F. Wien Langmuir 2018 34 (24) 7180-7191

"Reversible Morphological Control of Cholecystokinin Tetrapeptide Amyloid Assemblies as a Function of pH" F. Gobeaux*, F. Porcher, R. Dattani The Journal of Physical Chemistry B 2017 121 (14) 3059-3069
 

 

 

Peptide Nanotubes Molecular Control

Lanreotide nanotube suspension
observed by cryo-TEM.
"Directing Peptide Crystallisation through Curvature Control of Nanotubes" F. Gobeaux, C. Tarabout, N. Fay, C. Meriadec, M. Ligeti, D.-A. Buisson, J.-C. Cintrat, F. Artzner & M. Paternostre Journal of Peptide Science 2014 20 (7) 508-516

"Experimental Observation of Double-Walled Peptide Nanotubes and Monodispersity Modeling of the Number of Walls" F. Gobeaux, N. Fay, C. Tarabout, F. Meneau, C. Mériadec, C. Delvaux, J.-C. Cintrat, C. Valéry, F. Artzner, and M. Paternostre Langmuir 2013 29 (8) 2739−2745

"Structural Role of Counterions Adsorbed on Self-Assembled Peptide Nanotubes" F. Gobeaux*, N. Fay, C. Tarabout, M. Ligeti, D. Alexandre-Buisson, J.-C. Cintrat, K.M.H. Nguyen, C. Valéry, F. Artzner & M. Paternostre Journal of the American Chemical Society 2012 134 (1) 723-733

“Control of Peptide Nanotube Diameter by Chemical Modifications of an Aromatic Residue Involved in a Single Close Contact” C. Tarabout, S. Roux, F. Gobeaux, N. Fay, E. Pouget, C. Meriadec, M. Ligeti, M. IJsselstijn, F. Besselievre, D. Alexandre-Buisson, J.-M. Verbavatz, M. Petitjean, C. Valéry, L. Perrin, B. Rousseau, F. Artzner, M. Paternostre, J.-C. Cintrat Proceedings of the National Academy of Sciences of the United States of America  2011 108 (19) 7679-7684

 

 

 

 

Collagen self-assembly

 

Taken from Gobeaux et al. (2007)

“Power law rheology and strain-induced yielding in acidic solutions of type I-collagen” F. Gobeaux, E. Belamie, G. Mosser, P. Davidson, S. Asnacios Soft Matter 2010 6 3769-3777

"Fibrillogenesis in Dense Collagen Solutions: a Physico-chemical Study" F. Gobeaux, G. Mosser, A. Anglo, P. Panine, P. Davidson, M.-M. Giraud-Guille, E. Belamie Journal of Molecular Biology 2008 376 (5) 1509-1522

"Liquid Crystalline Properties of Type I Collagen: Perspectives in Tissue Morphogenesis" (Revue) M.-M. Giraud Guille, E. Belamie, G. Mosser, C. Hélary, F. Gobeaux, S. Vigier Comptes-Rendus Chimie 200811 245-252

"Cooperative Ordering of Collagen Triple Helices in the Dense State" F. Gobeaux, E. Belamie, G. Mosser, P. Davidson, P. Panine, M.-M. Giraud-Guille Langmuir 2007 23 (11) 6411-6417

"Possible Transient Liquid Crystal Phase during the Laying Out of Connective Tissues: α-Chitin and Collagen as Models" E. Belamie, G. Mosser, F. Gobeaux and M.-M. Giraud-Guille Journal of Physics: Condensed Matter 2006 18  (13) S115–S129

 

 

Biomineralization and mineral precipitation

"In vivo Inspired Conditions to Synthesize Biomimetic Hydroxyapatite" N. Nassif, F. Martineau, O. Syzgantseva, F. Gobeaux, M. Willinger, T. Coradin, S. Cassaignon, T. Azais, M.-M. Giraud-Guille Chemistry of Materials 2010 22 (12) 3653-3663

"Self-Assembled Collagen-Apatite Matrix with Bone-like Hierarchy" N. Nassif, F. Gobeaux, J. Seto, E. Belamie, P. Panine, P. Davidson, G. Mosser, P. Fratzl, M.-M. Giraud-Guille Chemistry of Materials 2010 22 (11) 3307–3309

Nanometrology/Analytical chemistry

"Contribution to Accurate Spherical Gold Nanoparticle Size Determination by Single-Particle Inductively Coupled Mass Spectrometry: A Comparison with Small-Angle X-ray Scattering" V. Geerstsen, E. Barruet, F. Gobeaux, J.-L. Lacour, O. Taché Anal. Chem. 2018 90 (16), 9742–9750

Microfluidics

Lysozyme crystals nucleated in
water-in-oil emulsion. 

"Elaboration of Materials with Functionality Gradients by Assembly of Chitosan-Collagen Microspheres Produced by Microfluidics" D. Azria, R. Guermache, S. Raisin, S. Blanquer, F. Gobeaux, M. Morille, E. Belamie Journal of Renewable Materials 2018 6 (3) 314-324

"Mapping and Manipulating Temperature-Concentration Phase Diagrams Using Microfluidics" S. Selimovic, F. Gobeaux, S. Fraden Lab on a Chip 2010 10 (13) 1696-1699

 

 

 

 

Miscellaneous

"Catalytically Active Peptides Affected by Self-Assembly and Residues Order" Avigail Baruch-Leshem, Corinne Chevallard, Frederic Gobeaux, Patrick Guenoun, Jean Daillant, Philippe Fontaine, Michel Goldmann, Ariel Kushmaro, Hanna Rapaport Colloids and Surfaces B: Biointerfaces 2021 111751

"Ligand-free synthesis of gold nanoparticles incorporated within cylindrical block copolymer films" F. Aubrit, F. Testard, A. Paquirissamy, F. Gobeaux, X. Wang, F. Nallet, P. Fontaine, V. Ponsinet, P. Guenoun J. Mater. Chem. C 2018 6 8194-8204

"Biocompatible Stimuli-Responsive W/O/W Multiple Emulsions Prepared by One-Step Mixing with a Single Diblock Copolymer Emulsifier" M. Protat, N. Bodin, F. Gobeaux, F. Malloggi, J. Daillant, N. Pantoustier, P. Guenoun, P. Perrin Langmuir 2016 32 (42) 10912-10919

"Calibration and quality assurance procedures at the far UV linear and circular dichroism experimental station DISCO" F. Wien, M. Paternostre, F. Gobeaux, F Artzner and M. Refregiers Journal of Physics: Conference Series 2013 425  122014

Pictures

 

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