Sophie COMBETLaboratoire Léon-Brillouin (LLB), Saclay, France +33 1 69 08 67 20 CV : CV_SCombet_feb19.pdf |
Staff scientist (CNRS) at the LLB, the French neutron source ; instrument responsible of PACE SANS (small-angle neutron scattering) diffractometer.
Coherent neutron scattering provides structural information in addition to, or instead of, the more classical structural methods used in structural biology (XR-crystallography, NMR, cryo-electron microscopy) in challenging situations such as protein assemblies or proteins in complex environments.
I use currently small-sangle neutron scattering (SANS) and specular neutron reflectivity (SNR) to probe:
Annighöfer B, Hélary A, Brûlet A, Colas de la Noue A, Loupiac C, Combet S. High pressure cell to investigate protein unfolding up to 600 MPa by small-angle neutron scattering. Rev Sci Instr, 90, 025106, 2019. DOI: 10.1063/1.5051765
Combet S, Cousin F, Rezaei H, Noinville S. Membrane interaction of off-pathway prion oligomers and lipid-induced on-pathway intermediates during prion conversion: a clue for neurotoxicity. Biochim Biophys Acta Biomembr 1861: 514, 2019. DOI: 10.1016/j.bbamem.2018.12.001
Dos Santos Morais R, Delalande O, Pérez J, Mias-Lucquin D, Lagarrigue M, Martel A, Molza AE, Chéron A, Raguénès-Nicol C, Chenuel T, Bondon A, Appavou MS, Le Rumeur E, Combet S, Hubert JF. Human dystrophin structural changes upon binding to anionic membrane lipid. Biophys J 115: 1231, 2018. DOI: 10.1016/j.bpj.2018.07.039
Chevreuil M, Law-Hine D, Chen J, Bressanelli S, Combet S, Constantin D, Degrouard J, Möller J, Zeghal M, Tresset G. Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte. Nature Comm 9: 3071, 2018. DOI: 10.1038/s41467-018-05426-8
Dos Santos Morais R, Delalande O, Pérez J, Mouret L, Bondon A, Martel A, Appavou MS, Le Rumeur E, Hubert JF, Combet S. Contrast-matched isotropic bicelles: a versatile tool to specifically probe the solution structure of peripheral membrane proteins using SANS. Langmuir 33: 6572-6580, 2017. DOI: 10.1021/acs.langmuir.7b01369
Le Coeur C, Combet S, Carrot G, Busch P, Teixeira J, Longeville S. Conformation of the polyethylene glycol chains in diPEGylated hemoglobin specifically probed by SANS: correlation with PEG length and in vivo efficiency. Langmuir 31: 8402-8410, 2015. DOI: 10.1021/acs.langmuir.5b01121
Combet S, Zanotti JM. Further evidence that hydration water is the main “driving force” of protein dynamics: a neutron scattering study on perdeuterated C-phycocyanin. Phys Chem Chem Phys 14: 4927-4934, 2012. DOI: 10.1039/c2cp23725c