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Univ. Paris-Saclay

Sujet de stage / Master 2 Internship

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Experimental study of the effect of the hydrodynamic flux on the adsorption of proteins at the surface of nanoparticles

Contact: GOBEAUX Frederic, , frederic.gobeaux@cea.fr, +33 1 69 08 55 21
Summary:
Upon introduction of nanoparticles in a biological medium, proteins adsorb on their surface, giving them a new biological identity that will define their bioactivity. The aim of this project is to study the effect of the hydrodynamic flow on this protein layer and on the nanoparticle stability.
Possibility of continuation in PhD: Oui
Deadline for application:28/02/2021

Full description:
Understanding the future of nanoparticles in biological media in of utmost importance for their potential use in biomedical applications. It is known that after in vivo administration of nanoparticles, their surface is rapidly covered by adsorbed proteins forming a so-called “corona” that strongly affects their biodistribution, pharmacokinetic, therapeutic efficacy and potential toxicity.1 While the structure and composition of this “protein corona” has already prompted a lot of research, the role of the hydrodynamic flow on the nanoparticle evolution in the presence of proteins (protein adsorption, nanoparticle aggregation or dissolution etc…) has so far received little attention although it is a key feature of blood medium.2
By combining different analytical techniques, we thus propose to develop a methodology to appreciate the effect of flow and shearing on the nanoparticles/proteins pair. Indeed, we will investigate the stability and integrity of the nanoparticles (scattering methods, cryo-TEM) as well as the conformational changes of the proteins (spectroscopic methods). When possible, these analyses will be performed in situ.
This project is carried out in collaboration with Simona Mura (Institut Galien, Faculté de Pharmacie de Chatenay Malabry and Frank Wien (Synchrotron Soleil). It is supported by the LabEx NanoSaclay.
Technics/methods used during the internship:
Circular dichroism, fluorescence spectroscopy, small angle x-ray/neutron scattering

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