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Sujet de stage / Master 2 Internship

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Development of lab on chip for the study of cancerous cells and/or bacterias

Contact: JASMIN-LEBRAS Guenaelle, , guenaelle.jasmin-lebras@cea.fr, +33 1 69 08 65 35
Summary:
The development of Lab on chips based on GMR sensors is a project of biotechnology for the health, for the diagnostic and therapeutic innovation. It is based on the combination of magnetic particles labeled with the specific antibody of the biological target and their dynamic detection with sensors. The real challenge is to obtain a lab on chip able to detect quickly, in a simple, sensitive and specific way, very low quantitty of biological objects , in answer to an emergency need for clinical diagnosis and/or for biosafety.
Possibility of continuation in PhD: Oui
Deadline for application:08/03/2018

Full description:
In this project, we are focusing on the detection of biological objects by using GMR sensors which allow a local detection of very weak magnetic fields.
The principle is to incorporate magnetic particles labeled with the proper specific antibody in a solution containing the biological fluid and to inject the solution in a microfluidic channel where sensitive magnetic sensors are placed underneath. A perpendicular magnetic field is magnetizing the magnetic particles and the stray dipolar field of the magnetic particles is detected. The signal produced by a labeled cell is much larger than magnetic nanoparticles and hence, it is possible to count single cells During the training period, in collaboration with a PhD-student, the student will optimize and will test the lab on chip integrating GMR sensors and a microfluidic channel made in clean room for which height varies according to the size of the studied biological objects. In collaboration with the biologists of the LERI Laboratoire d'Etudes et de Recherches en Immunoanalyse), he will label magnetic particles with the specific antibody of the biological target to detect. He will optimize the lab on chip as well as the various tests necessary to distinguish in a specific way these biological objects (cancer cells and/or bacteria) and to control the different involved parameters. He will analyze the results by developing simulations.
Technics/methods used during the internship:
Cleaning room technicals. , MBE technical, electronic. magnetic measurements

Laboratory
Tutor of the internship

 

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