CEA |   |   |   |   |   |   | webmail : intra - extra |  Accès VPN-SSL | Contact | Français

PhD subjects

2 sujets IRAMIS

Dernière mise à jour : 14-11-2018


««

• Soft matter and complex fluids

 

Biophysical and dynamical study of chromatin conformation during genome replication

SL-DRF-19-0435

Research field : Soft matter and complex fluids
Location :

Service Nanosciences et Innovation pour les Materiaux, la Biomédecine et l'Energie

Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire

Saclay

Contact :

Frédéric GOBEAUX

Patrick GUENOUN

Starting date : 01-10-2018

Contact :

Frédéric GOBEAUX

CEA - DRF/IRAMIS/NIMBE/LIONS

01 69 08 24 74

Thesis supervisor :

Patrick GUENOUN

CEA - DRF/IRAMIS/NIMBE/LIONS

01-69-08-74-33

Personal web page : http://iramis.cea.fr/Pisp/frederic.gobeaux/

Laboratory link : http://iramis.cea.fr/nimbe/lions/

The tridimensional organization of the genome and its dynamics in live cells are decisive to perform its functions. It is crucial to understand them and to identify the parameters controlling them. Current state of the art allows describing the short range (<10 nm) and long range (>250 nm) organization of chromatin conformation in the nucleus. However, there is an intermediate range (10-250 nm) where chromatin organization is difficult to apprehend. This range corresponds to the size of protein complexes that modify chromatin and harness genome replication.



We propose to monitor cell cultures during genome replication and other cellular events using small angle x-ray scattering. Thanks to a dedicated experimental set-up we will study chromatin conformation dynamics during genome duplication and complement this analysis with numerical simulations (molecular dynamics) so as to correlate chromatin dynamics with that of genome duplication. We will use different cell mutants and the addition of drugs to perturb the system and modify the observed structures.



This project is a collaboration between two teams of physicists and biologists and will consist for the student to reach a dual expertise in both disciplines.

Ultra-selective temperature-enhanced soft centrifuge separation in dynamic ultra-flexible microemulsion aggregates

SL-DRF-19-0339

Research field : Soft matter and complex fluids
Location :

Service Nanosciences et Innovation pour les Materiaux, la Biomédecine et l'Energie

Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire

Saclay

Contact :

Thomas ZEMB

Starting date : 01-10-2019

Contact :

Thomas ZEMB

CEA - DRF/ICSM

06 30 52 50 34

Thesis supervisor :

Thomas ZEMB

CEA - DRF/ICSM

06 30 52 50 34

Personal web page : http://www.icsm.fr/icsm_engl/cv-thzemb_en.html

Laboratory link : http://www.icsm.fr/

In order to move towards circular economy, energy savings as well as raw material recycling must be implemented as fast as possible. These new processes must produce low quantities of effluents as well as use as few quantities as possible surface active agents or other fluids that need to be recycled. The phD subject proposed is balanced in theory and in experimental part: its aim is a approach understanding the physical chemistry that is the basis of the processes to be designed based on first principle knowledge, and not systematic essays. For example is currently extremely difficult and too expensive in practice to separate lipids from proteins without torrefaction, sterols from pesticides from agricultural products or extract valuable/toxic synthons or byproducts from effluents in pharmacologic synthesis.



Recent observations made at ICSM on rare earth separation and refining using surfactant-free microemulsions have evidenced that fine-tuned separation are possible via soft centrifugation. The work proposed for this phD in physical chemistry is to compare expected yields and fluxes that can be derived from fundamental knowledge of the free energy involved in phase transitions at nano- and meso- scale. This will allow innovative and economically acceptable recycling processes without emulsifiers but based on soft centrifugation.



The knowledge gained by the student in the experimental approach as well as critical comparison to theoretical expressions proposed in the literature will be crucial to be able to design any advanced separation process, whatever the domain of application for which an enormous demand is expected in the next twenty years.

 

Retour en haut