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129Xe NMR-based biosensors @ LSDRM

A long-term collaboration exists with mainly two chemistry labs: ENS Lyon and CEA/IBITEC-S to conceive and study xenon 129Xe NMR-based biosensors. Several molecular systems for which xenon develops a particular affinity and that give the noble gas a specific resonance frequency well resolved from the bulk signal, have been chemically decorated with biological ligands

 

Biological targets

  • pH

Cancer, apoptosis              
    Chem. Eur. J. 16 (2010) 12941 DOI: 10.1002/chem.201001170

  • Metal ions

Cardiac diseases, diabetes, cancer, neurodegeneration         
    Angew. Chem. 51 (2012) 4100 DOI: 10.1002/anie.201109194
    Anal. Chem. 86 (2014) 1783 DOI: 10.1021/ac403669p

  • H2O2

Oxydative stress            
    Angew. Chem. 53 (2014) 9837 DOI: 10.1002/anie.201405349

  • DNA           

    ChemPhysChem 8 (2007) 2082 DOI: 10.1002/cphc.200700384

  • Cell surface receptors       

     Bioorg. Med. Chem. 19 (2011) 4135 DOI: 10.1016/j.bmc.2011.05.002

  • Recombinant proteins                       

    Chem. Comm. 51 (2015) 11482 DOI: 10.1039/C5CC04721H

 

 Measurement of pH

 

Local modification of pH is a key parameter in different pathologies  such as cancers or biological processes such as apoptosis.  We show that the sensitivity of xenon to its local environment and the presence of ionic groups on the host molecule can lead to interesting properties. For a first water-soluble cryptophane derivative, we show that a precise monitoring of the local pH can be performed. For a second cryptophane, the presence of ionic groups close to the cryptophane cavity modifies the xenon binding constant and in–out exchange rate. The latter allows the tuning of physical properties of xenon–cryptophane interactions without resorting to a change of the cavity size. These results open new perspectives on the influence of chemical modifications of cryptophanes for optimizing the biosensor properties.

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Detection of metal ions

 

Metal ions are involved in differents pathologies such as cardiac diseases, diabetes, cancer, neurodegeneration.  Here, an approach for sensitive magnetic resonance detection of metal cations is proposed. Combining the use of hyperpolarized 129Xe NMR and of a cage-molecule functionalized by a ligand able to chelate different cations, we show that simultaneous detection of lead, zinc, and cadmium ions at nanomolar concentration is possible in short time, thanks to fast MRI sequences based on the HyperCEST scheme.

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Detection of hydrogen peroxyde

 

Hydrogen peroxyde induces oxydative stress on cells, involved in the development of pathologies such as cancers, heart failures, Parkinson's disease.  We propose here a probe able to detect H202 using 129Xe NMR.

Understanding the nature of host–guest interactions in supramolecular complexes involving heavy atoms is a difficult task. Described herein is a robust simulation method applied to complexes between xenon and members of a cryptophane family. The calculated chemical shift of xenon caged in a H2O2 probe, as modeled by quantum chemistry with complementary-orbital, topological, and energy-decomposition analyses, is in excellent agreement with that observed in hyperpolarized 129Xe NMR spectra. This approach can be extended to other van der Waals complexes involving heavy atoms.

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Detection of a specific DNA strand

 

A xenon host composed of a cryptophane structure with a DNA strand serves to detect its complementary strand in the micromolar range through laser-polarized 129Xe NMR spectroscopy.

 

 

Detection of a cell surface receptor: the transferrin case

For detection of biological events in vitro, sensors using hyperpolarized 129Xe NMR can become a powerful tool, provided the approach can bridge the gap in sensitivity. Here we propose constructs based on the non-selective grafting of cryptophane precursors on holo-transferrin. This biological system was chosen because there are many receptors on the cell surface, and endocytosis further increases this density. The study of these biosensors with K562 cell suspensions via fluorescence microscopy and 129Xe NMR indicates a strong interaction, as well as interesting features such as the capacity of xenon to enter the cryptophane even when the biosensor is endocytosed, while keeping a high level of polarization. Despite a lack of specificity for transferrin receptors, undoubtedly due to the hydrophobic character of the cryptophane moiety that attracts the biosensor into the cell membrane, these biosensors allow the first in-cell probing of biological events using hyperpolarized xenon.

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Recombinant proteins 

Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized 129Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the 129Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association

 

Maj : 24/02/2017 (2708)

 

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